Application

Western blottingDilution 1:1000Tissue/Cell Type Used: monkey COS-Beta-4-Spectrin-HisImmunofluorescenceDilution 1:100Tissue/Cell Type Used: SK-N-BE

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Immunogen

Immunogen Species: Human

Immunogen Description: Fusion protein amino acids 1621-1832 (C-terminal repeats 14 to 15) of human Beta4-spectrin

Immunogen type: Fusion protein

Immunogen Region: C-terminal

Other Notes

Additional Product Details:Does not cross react with other Beta-spectrinsLot Expiration/Retest Date: Expires 1 year from purchase

Target/Gene Description: Spectrin is the major constituent of the cytoskeletal network underlying the erthrocyte plasma membrane and determines cell shape, arrangement of transmembrane proteins and organization of organelles. It is expressed at very low levels in many tissues, with the strongest expression in the cerebellum, spinal cord, stomach, pituitary gland, liver, pancreas, salivary gland, kidney, bladder and heart . Spectrin beta 4 is a non-erythrocyte member, and is expressed in the brain and pancreatic islets and localisted to the nuclear matrix, cytoplasmic vesicles and PML nuclear bodies. Beta4 spectrins are also essential for membrane stability and the molecular organization of the nodes of Ranvier .Purification method: Protein G purifiedProduct type: Primary antibodyAntibody structure: Whole AntibodyProtein ID: NP_066022.2

Physical form

50% glycerol, 0.1% sodium azide

PBS pH 7.4

Preparation Note

Derived from animal serum

Storage and Stability

Protect from light; avoid repeated freeze-thaw cycles. Store at -20 °C long-term, 2-8 °C short-term.